rabbit primary antibody against glut4  (ABclonal Biotechnology)

Journal: Nature Metabolism

Article Title: Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise

doi: 10.1038/s42255-024-01153-1

Figure Lengend Snippet: a , Graphical representation of the EHC. b , GIR during the insulin clamp. c – e , GIR ( c ), endogenous glucose rate of appearance (RA) ( d ) and glucose rate of disappearance (RD) ( e ) at 120 min of the clamp. f , Plasma FAs during the clamp. g , Average leg glucose uptake during the last 40 min of the clamp. h , Targeted immunoblotting analyses of proteins associated with glucose metabolism, fat metabolism and the mitochondrial electron transport chain in skeletal muscle. i , Volcano plot showing proteome log 2 fold change (FC) (carriers/controls) plotted against –log 10 P value highlighting downregulated proteins TBC1D4, TBC1D1 and GLUT4 (SLC2A4). *Difference between TBC1D4 carriers and controls at the given time point. ‡Different from basal (time 0) within the same group. n = 5 ( b – g , i ). n = 7 ( h ) except for mTOR, p70S6K and ACC ( n = 5 in controls and TBC1D4 carriers) as well as Akt2, AMPKα2, CS, CD36 and FATP4 ( n = 6) in controls and GLUT1 ( n = 6) in TBC1D4 carriers. Data are means ± s.e.m. Data were analysed using a two-tailed paired Student’s t -test ( c – e , g ), a two-tailed non-paired Student’s t -test ( h ) as well as a two-way repeated ANOVA test (two-factor repeated) and two-tailed Student–Newman–Keuls post hoc analyses for multiple comparisons ( b , f ). Graphics in a created using BioRender.com . a.u., arbitrary units.

Article Snippet: For staining, four to six individual fibres were teased out from the fixed fibre bundles with fine forceps and blocked for 2 h in PBS containing 5% goat serum, 1% BSA and 0.04% saponin and then incubated overnight with primary antibody raised against GLUT4 (rabbit polyclonal; no. PA5-23052; lot WJ3403509, Thermo Scientific).

Techniques: Western Blot, Two Tailed Test

Journal: Nature Metabolism

Article Title: Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise

doi: 10.1038/s42255-024-01153-1

Figure Lengend Snippet: a , Representative immunoblots for data related to figure panel 2 h. b , c , Distribution of Myosin Heavy Chains (MHC) ( b ) and regulatory metabolic ( c ) proteins in MHC-defined type 1 and type 2 skeletal muscle fiber bundles. d , Representative immunoblots. e , Quantification of GLUT4 imaging by confocal microscopy of isolated skeletal muscle fibers. n = 7 in Controls and n = 6 in TBC1D4 carriers ( b , c ). n = 15 fibers obtained from 3 subjects (4 to 6 fibers/subject) in each group ( e ). Data are means ± SEM. Data were analyzed using a two-tailed non-paired students t-test ( e ) as well as a two-way repeated ANOVA test (one factor repeated) and two-tailed Student-Newman-Keuls post hoc analyses for multiple comparisons ( b , c ). AU, arbitrary units. Scale bar in panel e is 10 µm.

Article Snippet: For staining, four to six individual fibres were teased out from the fixed fibre bundles with fine forceps and blocked for 2 h in PBS containing 5% goat serum, 1% BSA and 0.04% saponin and then incubated overnight with primary antibody raised against GLUT4 (rabbit polyclonal; no. PA5-23052; lot WJ3403509, Thermo Scientific).

Techniques: Western Blot, Imaging, Confocal Microscopy, Isolation, Two Tailed Test